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membrane type of cell culture filtration


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3 replies to this topic

#1 sara.r

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Posted 05 January 2010 - 06:31 AM

hi
there are different kinds of membranes in 0.2 micrometer syringe filters, which one is recommanded for filterind cell culture solutions and media?
regards

#2 mostafa

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Posted 05 January 2010 - 07:43 AM

Hi
I think Milipore is good

good luck

#3 bob1

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Posted 05 January 2010 - 03:24 PM

Sara... use one with a low protein binding capability - you don't want to remove the precious proteins and amino-acids from your medium.

#4 rhombus

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Posted 06 January 2010 - 06:30 AM

hi
there are different kinds of membranes in 0.2 micrometer syringe filters, which one is recommanded for filterind cell culture solutions and media?
regards



We generally use Cellulose Acetate memrane filters. As bob1 correctly states, any membrane that has low protein binding, as you do not want to lose precious growth factors.

I do however find it strange that when media, FCS, PEN/STEP, NEAA etc all come sterile...why would you want to filter it.

If you are adding drugs/compounds into your TC media, I have always found it best to make them up in a concentrated solution....then filter sterilise that.

Hope this is useful.

Kindest regards.

Uncle rhombus




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