Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

dilution problem


  • Please log in to reply
1 reply to this topic

#1 whiskey87

whiskey87

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 04 January 2010 - 06:48 PM

hey guys,

I was hoping someone could help me with the following problem:

I have lyophilized oligonucleotide (primer), which is 37885 Picomoles and I want to make it 100 micromolar final concentration. Could someone kindly take the time to explain how to go about doing this.

Regards,

AF

#2 Rob Steuart

Rob Steuart

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 04 January 2010 - 11:33 PM

Easiest way is to take the number of nanomoles multiply this by ten then add that amount in microliters of PCR grade water or TE.

For example you have 37885 picomoles which is 37.885 nanomoles. Multiply that by ten and you get 378.85. Therefore you need to add 378.85 microliters of liquid to your lyophilised primer




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.