How do i increase my rna yield
Posted 04 January 2010 - 03:35 PM
If anyone had any other ideas as to how to improve total amount of RNA. In combining all the aliquots together i wont lose any RNA will i?, but i could change the quality (I check all the RNA on an agilent PICO chip (RINs are between 5.7-6.9).
Could i post cleanup this entire pool that i make? Is there a good protocol for this. Again would i lose RNA?
Posted 04 January 2010 - 06:53 PM
Posted 06 January 2010 - 04:34 PM
Im using an rneasy plus mini column to extract rna from my sorted cells.I only get approx 200ng rna from my sorted cells. I need to get around 1-5ug for sequenicng. Is it best to combine the rna aliquots asnd then clean this up or combine the sorted cells and do one big rna extraction
How are you preparing the RNA....RNeasy, Trizol, other? and from what source...tissues, cells, plants, soil etc. Need more info to be of much help
Posted 07 January 2010 - 12:28 AM