
Protein G vs Protein A for Rabbit Antibodies
#1
Posted 04 January 2010 - 08:28 AM
Thanks
MM
#2
Posted 04 January 2010 - 03:30 PM
#3
Posted 04 January 2010 - 07:51 PM
#4
Posted 05 January 2010 - 08:06 AM
#5
Posted 06 January 2010 - 05:43 AM
MM
#7
Posted 07 January 2010 - 12:19 PM
If I get any noticeable differences with using protein A for protein G beads I'll be sure to post for anyone else that may have this question.
MM
#8
Posted 26 January 2010 - 09:50 PM
Thanks, I've seen this from invitrogen. However, I've seen some other stuff (e.g, the book: Using Antibodies: A laboratory manual by Ed Harlow and David Lane) that suggests one should use protein A for rabbit antibodies during immunoprecipitation. Hence my confusion I suppose, different sources have different suggestions and I was wondering if anyone had any experience with this issue.
If I get any noticeable differences with using protein A for protein G beads I'll be sure to post for anyone else that may have this question.
MM
MM, can I know from where you get your protein A beads from? i would like to try using protein A beads too since i dont get much DNA enrichment after using Protein G beads. Thank you very much
#9
Posted 26 January 2010 - 10:16 PM