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Supercoil vs Open Circular forEtBr intercalating

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#1 anonymous



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Posted 05 April 2001 - 09:00 PM

I've been using the 2d electrophoresis, but i've not been able to get a good similarity pattern when I use 2d-standads. What standards conditions to use, for capillar making, running conditions and very important: what steps can be postponned, because a whole process take so much time? fINALLY I've monitored pH during a experiment at 500V, but the gradient keeps OK only untill 1,5 hrs

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