I've been using the 2d electrophoresis, but i've not been able to get a good similarity pattern when I use 2d-standads. ¿ What standards conditions to use, for capillar making, running conditions and very important: what steps can be postponned, because a whole process take so much time? fINALLY I've monitored pH during a experiment at 500V, but the gradient keeps OK only untill 1,5 hrs
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Supercoil vs Open Circular forEtBr intercalating
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