Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

EDTA bacterial cell lysate


  • Please log in to reply
2 replies to this topic

#1 Danny Chow

Danny Chow

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 03 January 2010 - 06:52 PM

Does anybody using EDTA to lysate bacterial cell ?

Could you please provide me the lysate buffer ?

i am using 2 buffers

1) Tris/Cl 20mM, EDTA 2.5mM, sucrose 20% (30mins on ice) 10ml for 1L bacterial culture

2) Tris/Cl 20mM, EDTA 2.5mM (30 mins on ice) 10ml for 1L bacterial culture

I will discard the (1) solution, and then centrifuge down the pellet, then add (2)
I found that almost 50% of the target protein was appeared in the (1)

Thanks

#2 aimikins

aimikins

    Sunflower

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 117 posts
2
Neutral

Posted 04 January 2010 - 03:31 PM

what kind of bacteria?
"it is a miracle that curiosity survives formal education" -A.E.

#3 Danny Chow

Danny Chow

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 12 January 2010 - 07:50 PM

what kind of bacteria?


E.Coli




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.