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PCR troubleshooting


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4 replies to this topic

Poll: hi adrian (0 member(s) have cast votes)

sorry i don't have the pic on my PC right now,,but it's two clear bands even in the control template.most probably primer dimer, i used DMSO, 8.5%, becuase my primer has high G,C, content, plz help

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#1 maxmix

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Posted 02 January 2010 - 11:23 PM

hi all:
im new in molecular bilogy, i had amplified DNA product by primer that has high G,C content designated by garovic et al.2003. to get 107bp, i run the agrose gel 2.5%, then i got two band in the expected site,,,why two bands? plz help :P
max,,

#2 Michaelro

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Posted 03 January 2010 - 09:15 AM

Hi
Most probably You have a primer dimers.
Do You see one of the bands on no template control?

#3 Adrian K

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Posted 03 January 2010 - 09:40 AM

hi max,
can you attach your picture?
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#4 maxmix

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Posted 03 January 2010 - 07:42 PM

Hi
Most probably You have a primer dimers.
Do You see one of the bands on no template control?



#5 maxmix

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Posted 03 January 2010 - 07:46 PM

hi max,
can you attach your picture?






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