Expired conjugated Antibodies?
Posted 02 January 2010 - 07:53 AM
I'm just wondering when conjugated ab's are too old to use? In my lab there's a big box with lots of expired ab's, and I would like to test some of them to see if my cells of interest express that particular marker at all.
I have antibodies that expired just one or two years ago and they works fine, but what about e.g. five years?
What will likely be the major problem with these old reagents? Decreased binding capacity or decreased fluorescence capacity?
Posted 02 January 2010 - 08:06 AM
Posted 03 January 2010 - 08:53 AM
According to several manufacturers, ab's should probably be useless after a couple of years, but now I doubt that.
Posted 05 January 2010 - 05:41 PM
Of course some of those antibodies really were dead . Tandem-dye antibodies are the least stable-not a big surprise!
Posted 06 January 2010 - 12:40 AM