I am using a UV gel documentation system to visualize ethidium bromide-stained DNA. For some reason I need to look directly at the gel while being exposed to UV ( for a short period of course ) . For that , I need UV resistant goggles, but we don't currently have them in the lab because we used to rely on the camera attached to the system . However , we do have plastic glasses for protection against chemicals etc . I am thinking that since Plastic ( Polycarbonate ) is known to be opaque to UV so I can use them instead. I've done a little experiment to support my assumption. I actually managed to put the glasses in the light path of a UV spectrophotometer, closed the lid and read the absorption at 260nm and it was infinity.
So what would you think?
Edited by Bassaml7, 01 January 2010 - 02:26 AM.