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isolation of RNA from insect cells


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#1 sowmi

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Posted 30 December 2009 - 10:24 PM

Hi all..

I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.

thanks in advance.

#2 klinmed

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Posted 31 December 2009 - 05:55 AM

Hi all..

I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.

thanks in advance.

A large number of methods have been described including those using commercially available kits (Trizol and RNAeasy for instance). Google "lepidopteran rna extraction".

Hope this helps

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#3 sowmi

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Posted 31 December 2009 - 10:41 PM

Thanks klinmed
I found few papers.. and I am working on the protocol..




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