Hi all..
I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.
thanks in advance.
isolation of RNA from insect cells
Started by sowmi, Dec 30 2009 10:24 PM
2 replies to this topic
#1
Posted 30 December 2009 - 10:24 PM
#2
Posted 31 December 2009 - 05:55 AM
sowmi, on Dec 31 2009, 07:24 AM, said:
Hi all..
I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.
thanks in advance.
I am about to start RNA isolation from insect cells. I could not find a satisfactory protocol for this purpose. Can someone suggest a protocol to start with? I will also be doing some DNA isolations. My insect is a Lepidopteran and I plan to do the nucleic acid isolation from the larval stages.
thanks in advance.
A large number of methods have been described including those using commercially available kits (Trizol and RNAeasy for instance). Google "lepidopteran rna extraction".
Hope this helps
Attached File(s)
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1471_2164_7_160.pdf (1.36MB)
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#3
Posted 31 December 2009 - 10:41 PM
Thanks klinmed
I found few papers.. and I am working on the protocol..
I found few papers.. and I am working on the protocol..













