I extract RNA from plant leaves tissue using trizol protocol, my RNA purity OD260/280 around 1.5, some 1.48 but OD260/230 from 2.2-2.6. What happen with my samples ? How can i get higher OD260/280 ?. Thank so muck !!!
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#2
pDNA
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Posted 31 December 2009 - 12:12 AM
you have to get rid of traces of phenol and proteins!
Try another round of phenol:chloroform:isoamylalcohol extractions, this should improve your purity if it is that important for your downstream applications.
When doing Trizol extraction try not to get in contact with the interphase!
Regards,
p
Try another round of phenol:chloroform:isoamylalcohol extractions, this should improve your purity if it is that important for your downstream applications.
When doing Trizol extraction try not to get in contact with the interphase!
Regards,
p
#3
Vini
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Posted 31 December 2009 - 02:44 AM
hey, sometimes column purification also helps....
