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how to get higher RNA purity?


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#1 hientrung_pth

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Posted 30 December 2009 - 06:30 PM

I extract RNA from plant leaves tissue using trizol protocol, my RNA purity OD260/280 around 1.5, some 1.48 but OD260/230 from 2.2-2.6. What happen with my samples ? How can i get higher OD260/280 ?. Thank so muck !!!

#2 pDNA

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Posted 31 December 2009 - 12:12 AM

you have to get rid of traces of phenol and proteins!

Try another round of phenol:chloroform:isoamylalcohol extractions, this should improve your purity if it is that important for your downstream applications.

When doing Trizol extraction try not to get in contact with the interphase!

Regards,
p

#3 Vini

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Posted 31 December 2009 - 02:44 AM

hey, sometimes column purification also helps....

#4 hientrung_pth

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Posted 02 January 2010 - 02:26 AM

Thanks so much.




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