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single cell DNA isolation frommammalian cells


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#1 anonymous

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Posted 03 August 2001 - 09:00 PM

Does anyone have a reliable protocol for isolating DNA from a single mammalian cell?

#2 anonymous

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Posted 07 August 2001 - 09:00 PM

What do you actually mean from a single mammalian cell?

#3 anonymous

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Posted 07 August 2001 - 09:00 PM

Yes Dimitris...from one single cell.

#4 anonymous

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Posted 07 August 2001 - 09:00 PM

What do you mean from a single mammalian cell? Just one cell?

#5 anonymous

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Posted 22 August 2001 - 09:00 PM

I will suggest the protocol I know that can be used for single sperm cells. I believe it is easily adapted to somatic cells. PNAS 95:10791. The protocol involves embedding in agarose, scraping single cells off with a blade, lysing, neutralizing and then using the sample for PCR.




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