I run the 9% big gel overnight at 50V, but high molecular protein and marker disappeared, lower molecular proteins are ok.
what is the problem? running buffer?
any suggestion will be appreciated!
happy holidays.
Michael
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1 votes
disappearing high molecular protein and marker,thanks
Started by michaelzhang, Dec 29 2009 11:10 AM
1 reply to this topic
#2
mdfenko
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an elder
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Excellent
Excellent
Posted 30 December 2009 - 06:44 AM
how high mw? are you sure they entered the gel?
maybe they are diffuse.
how do you visualize (coomassie, silver, immunostain)?
is there enough to detect?
maybe they are diffuse.
how do you visualize (coomassie, silver, immunostain)?
is there enough to detect?
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
