Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Transwell co-culture and thymidine proliferation assay


  • Please log in to reply
2 replies to this topic

#1 zuzuk

zuzuk

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 28 December 2009 - 08:40 PM

Hi guys,

I have a problem. Both my cells are adherent cell, BV2 and MSC.
I have to determine the proliferation of BV2 after co-culture with MSC.
However, because both cells are adherent cells, i need to separate both cells with transwell.
Transwell only can fits into 6 well, 12 well and 24 well plate.
Meanwhile, proliferation assay must be prepared in 96 well plate! :)
Should I,
1. trypsinize the cell and transfer from 12 well plate to 96 well plate?
2. is there anyhow i should do? :angry:

Thank you :D

#2 jah

jah

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 44 posts
0
Neutral

Posted 29 December 2009 - 10:04 AM

Why must the proliferation assay be done in a 96-well plate? seems that you could scale-up

#3 zuzuk

zuzuk

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 29 December 2009 - 07:31 PM

because the machine only can read 96 well plate B)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.