when performed receptor binding assay using astrocyte cell pellet and 3H-DPCPX, i chose CPA as the displacer. and then the nonspecific binding is about 80% of the total.
it happened 3 times, so i thought maybe the CPA concentration was not high enough. so i did 9 different concentration of CPA (from the highest permitted solubility, 10 time gradient dilution), and used the fixed concentration of 3H-DPCPX. but the nonspecific binding was still about 80%-120% of the total.
i would highly appreciate your insights!!
and happy holiday!
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nonspecific binding is higher than total binding in radioligand binding assay, W
Started by bluejar, Dec 28 2009 12:13 PM
2 replies to this topic
#2
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Posted 07 January 2010 - 11:13 AM
Can you please clarify (name and structure)
3H-DPCPX
and
CPA
thank you.
3H-DPCPX
and
CPA
thank you.
#3
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Posted 18 January 2010 - 08:23 AM
sure! and thanks for your reply.
3H-DPCPX is a radioactive ligand, with a structure can be found in the following link:
http://www.iuphar-db.org/DATABASE/LigandDi...rd?ligandId=386
CPA(N6-Cyclopentyladenosine) is used as the displacer to cover all the non-specific binding from A1R in this experiment. the structure of CPA can be found here:
http://www.sigmaaldrich.com/catalog/Produc..._KEY&F=SPEC
or:
http://www.iuphar-db.org/DATABASE/LigandDi...rd?ligandId=435
3H-DPCPX is a radioactive ligand, with a structure can be found in the following link:
http://www.iuphar-db.org/DATABASE/LigandDi...rd?ligandId=386
CPA(N6-Cyclopentyladenosine) is used as the displacer to cover all the non-specific binding from A1R in this experiment. the structure of CPA can be found here:
http://www.sigmaaldrich.com/catalog/Produc..._KEY&F=SPEC
or:
http://www.iuphar-db.org/DATABASE/LigandDi...rd?ligandId=435
sgt4boston, on Jan 7 2010, 03:13 PM, said:
Can you please clarify (name and structure)
3H-DPCPX
and
CPA
thank you.
3H-DPCPX
and
CPA
thank you.
