Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is an enzyme protein of 1100 aa too big to be expressed in E coli


  • Please log in to reply
4 replies to this topic

#1 clwop

clwop

    member

  • Active Members
  • Pip
  • 11 posts
1
Neutral

Posted 27 December 2009 - 05:39 PM

i want to express a enzyme gene about 3500bp (fusing two subunits) from Corynebacterium glutamicum in E. coli. It is almost twice as big as the enzymes i usually deal with. so i wonder if its size will cause some trouble.

Edited by clwop, 27 December 2009 - 05:41 PM.


#2 Vini

Vini

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 178 posts
1
Neutral

Posted 27 December 2009 - 07:16 PM

No, the size will not be a problem.....people express proteins as big as 400 kDa from E. coli............so u dont have to worry.... :rolleyes:

#3 clwop

clwop

    member

  • Active Members
  • Pip
  • 11 posts
1
Neutral

Posted 27 December 2009 - 10:42 PM

No, the size will not be a problem.....people express proteins as big as 400 kDa from E. coli............so u dont have to worry.... :rolleyes:

thx

#4 Prep!

Prep!

    Am I me???!!!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 517 posts
4
Neutral

Posted 27 December 2009 - 11:25 PM

No, the size will not be a problem.....people express proteins as big as 400 kDa from E. coli............so u dont have to worry.... :rolleyes:

thx



http://mcl1.ncifcrf....c-Esposito.html

please refer here... it may not be impossible.. but expressing large mol wt protein in E Coli may be tricky...
Support bacteria - They are the only culture some people have!!!
Cheers!!!

#5 Vini

Vini

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 178 posts
1
Neutral

Posted 27 December 2009 - 11:39 PM

protein expression can be tricky for varied reasons.................not just the size............dont worry, we routinely express such proteins in our lab.............they may be unstable but take precautions like maintaining 4 degree, adding protease inhibitors if required and dont make it undergo many freeze-thaw cycles..............

Edited by DRN, 28 December 2009 - 12:32 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.