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Sequence Specific Oligonucleotide Analysis, Interpretation

Started by Mazhar Hussain, Dec 25 2009 01:34 AM

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2 replies to this topic

#1 Mazhar Hussain

Virion

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Posted 25 December 2009 - 01:34 AM

Hi, Dear Friends,
I am bit confuse about Sequence Specific Oligonucleotide Analysis...
Principle Says,
By "Mismatched" heteroduplex formation, it will cause its speed on the Acryamide gel slow as compared to normal band...
I have to Ask following questions,

1. In such analysis which PCR variant is used for DNA amplification?
2. If Fragment "mismatched" used to be slow as shown in lane 1 and 4 of following figure, then why this didn't heppen in lane 2 and 5? (I think this band was to appear beside the upper band)

Thank You very much in advance for paying keen attention on this problem....

Attached Files

Mazhar11111.bmp 768.63K 54 downloads

Mazhar Hussain
Microbiology & Molecular Genetics
Website: Microbiology On-Line
FaceBook Group: Microbiology On-Line

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#2 Adrian K

Legendary Graduate Beggar

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684 posts

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Excellent

Posted 27 December 2009 - 07:40 AM

1. No full paper, hard to tell.

2. This is not mismatch, this is deletion.

Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434