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[Mazhar Hussain]

Hi, Dear Friends,
I am bit confuse about Sequence Specific Oligonucleotide Analysis...
Principle Says,
By "Mismatched" heteroduplex formation, it will cause its speed on the Acryamide gel slow as compared to normal band...
I have to Ask following questions,

1. In such analysis which PCR variant is used for DNA amplification?
2. If Fragment "mismatched" used to be slow as shown in lane 1 and 4 of following figure, then why this didn't heppen in lane 2 and 5? (I think this band was to appear beside the upper band)

Thank You very much in advance for paying keen attention on this problem....

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