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#1 ujla80

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Posted 24 December 2009 - 01:09 AM

hi all :D

i had to store my serum samples at -80 but due to some reasons i had to store them at -20. now the problem is solved (after a week) & i can store them at -80. i have to analyse cytokines (by ELISA) after 5-6 mo... is it ok to store them at -80 now??does the transfer make any difference to the sample?
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#2 Gerard

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Posted 24 December 2009 - 04:09 AM

I think is very hard to tell the effects of storage conditions at the results, first the different cytokines will have different stability charisterisks under the same conditions and in the studys that describe stability are stable conditions used.
Storing at -80 will help but can not prevent degrading of the cytokines.
My advice would be store at -80 as soon as possible and also analyse the samples as soon as possible.

A study like in the link will give you some insight in the problems.
http://www.biomedcen...1471-2172/10/52

In the litterature there are much more to find.
Ockham's razor
Pluralitas non est ponenda sine necessitate
-- "You must assume no plural without necessity".

#3 ujla80

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Posted 24 December 2009 - 05:57 AM

thank you very much <_< the study link has really helped me a lot.




I think is very hard to tell the effects of storage conditions at the results, first the different cytokines will have different stability charisterisks under the same conditions and in the studys that describe stability are stable conditions used.
Storing at -80 will help but can not prevent degrading of the cytokines.
My advice would be store at -80 as soon as possible and also analyse the samples as soon as possible.

A study like in the link will give you some insight in the problems.
http://www.biomedcen...1471-2172/10/52

In the litterature there are much more to find.


god is great

#4 ujla80

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Posted 24 December 2009 - 06:00 AM

and what if blood is hemolysed wont it interfere with the detection?


I think is very hard to tell the effects of storage conditions at the results, first the different cytokines will have different stability charisterisks under the same conditions and in the studys that describe stability are stable conditions used.
Storing at -80 will help but can not prevent degrading of the cytokines.
My advice would be store at -80 as soon as possible and also analyse the samples as soon as possible.

A study like in the link will give you some insight in the problems.
http://www.biomedcen...1471-2172/10/52

In the litterature there are much more to find.


god is great




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