Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

culture medium


  • Please log in to reply
1 reply to this topic

#1 wuxx0153

wuxx0153

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 35 posts
1
Neutral

Posted 23 December 2009 - 05:54 PM

We would like to study the effect of some growth factors in cell culture.
I think an important step is to remove those growth factors from medium.

Can anyone give some suggestion on how o remove those growth factors from medium, or we should pay for the company to get those growth factors free medium.

#2 jah

jah

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 44 posts
0
Neutral

Posted 24 December 2009 - 07:29 AM

Serum-free media would accomplish this. Basal media do not have any GF's, just salts, vitamins, amino acids, glucose, and occasionally nucleosides. You can then build your specific 'defined' media formulation upon this base


In my serum free experiments, I add ITS+ supplement from BD which contains Insulin, BSA, apo-transferrin, and sodium selenite and simple fatty acids, and then add whatever GF I'm interested in studying.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.