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Does RT step smplify RNA


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#1 huzazhang

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Posted 23 December 2009 - 03:33 PM

I am doing a 2 step rt pcr, and I was wondering the first step, where I use superscript II reverse transcriptaser to reverse transcribe the rna into cdna (42C for 1 hr). Does this step amplify the cDNA produced at all or does it just create the same number ofcdna as the origional rna?


Cheers!

HUz

Edited by huzazhang, 23 December 2009 - 03:35 PM.


#2 phage434

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Posted 23 December 2009 - 04:14 PM

Same number (ideally) of cDNA molecules as the initial number of mRNA copies.

#3 huzazhang

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Posted 23 December 2009 - 04:20 PM

thanx




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