2 replies to this topic

[huzazhang]

I am doing a 2 step rt pcr, and I was wondering the first step, where I use superscript II reverse transcriptaser to reverse transcribe the rna into cdna (42C for 1 hr). Does this step amplify the cDNA produced at all or does it just create the same number ofcdna as the origional rna?


Cheers!

HUz

Edited by huzazhang, 23 December 2009 - 03:35 PM.

[phage434]

Same number (ideally) of cDNA molecules as the initial number of mRNA copies.

[huzazhang]

thanx