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low 260/280 ratio

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#1 flashboy



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Posted 19 February 2002 - 06:37 AM

i regularly extract rna from human islets [notoriously bad for rna extractions] and i use 0.3M sodium acetate and LPA carrier to aid in the precipitation step.

My problem is that although i have a 30 min incubation in DNase at 37 degrees, i get 260/280 ratios of below 1.5.  I have heard that the sodium acetate can interfere with the ratio, can anybody suggest how i may solve this problem.

#2 Meihua101



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Posted 22 February 2002 - 12:57 PM

Did you do phenol-chloroform extraction again after Dnase?

If you are short in RNA, you can use the DNase inhibitor instead of phenol-chloroform extraction

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