
Does anyone out there use it regularly?!
I've checked the literature. I've searched google. I've spoken with tech support at Sigma. I can't find any information regarding the typical storage conditions of 3-AT. I've got it at 1 M in water, filter sterilized and stored at -20. Is this pretty standard? How long is it good stored at -20? Should I be making small aliquots to avoid freeze-thawing? I thaw it in a room temperature water bath. Should I be making it up fresh? For the plates I'm making, I add the 3-AT to a solution of non-autoclavable reagents, filter sterilize, and bring it up to about 50 C. I then add that solution to an autoclaved agar solution that's at about 50-60 C. Is this OK? Is 3-AT stable at 50 C? For how long?
Any info would be GREATLY appreciated!!
