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Restriction Enzymes


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#1 rocketfan86

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Posted 21 December 2009 - 02:59 PM

Our lab does not have many restriction enzymes and i was wondering if an enzyme from Invitrogen and one from NEB can work using NEB Buffers?

#2 bob1

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Posted 21 December 2009 - 03:35 PM

Check the composition of the buffers, but many will work well together - note that different REs often have different buffers.

#3 molgen

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Posted 22 December 2009 - 12:33 AM

I have many RE from various source and usually don't have a problem with mix and matching. The only time it really was an issue, what I ended up doing is looking at the buffer composition and after the first digestion I added only the missing components from the buffer of the next digestion.
Make up a stock solution of 10 fold of the missing components and add it to the reaction buffer.
Good luck




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