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RNA recovery from Ethanol


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#1 Jka83

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Posted 21 December 2009 - 03:34 AM

Hey

I have RNA in ethanol in -70. Its been there for years.
I wanto to run these RNA samples with RT-PCR so I have to recover the RNA.
I centrifuge the sample (4C, 30 min,13000 rpm) and evaporate the residual ethanol. After that Ill add water. Would 20ul of water be good or?

#2 madrius1

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Posted 21 December 2009 - 09:42 AM

1 - Have you precipitated your RNA first?
2 - The volume in witch you will resuspend your RNA is quite a bit dependant of the quantity of RNA you have.. But 20 l is a good starting point.

#3 Curtis

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Posted 21 December 2009 - 08:55 PM

yeah, 20 ul should be fine if the conc is in a good range. we usually add 30 ul. use RNase free tips.




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