RNA recovery from Ethanol
Posted 21 December 2009 - 03:34 AM
I have RNA in ethanol in -70. It´s been there for years.
I wanto to run these RNA samples with RT-PCR so I have to recover the RNA.
I centrifuge the sample (4C, 30 min,13000 rpm) and evaporate the residual ethanol. After that I´ll add water. Would 20ul of water be good or?
Posted 21 December 2009 - 09:42 AM
2 - The volume in witch you will resuspend your RNA is quite a bit dependant of the quantity of RNA you have.. But 20 µl is a good starting point.
Posted 21 December 2009 - 08:55 PM