1. normal, untreated cell
2. cell treated with transfection reagent only
3. cell transfected with validated negative control siRNA (transfection reagent+ nc siRNA)
Different paper reported data with different control, some papers used 1 & 3, while some used 2 & 3.
can somebody tell me which control to be included, so that relative density (relative remaining protein expression) can be carried out? To which control should I compare with?
Thank a lot!
Edited by dcch, 21 December 2009 - 03:36 AM.