Posted 24 July 2002 - 12:37 AM
did anyone ever did ligation of 3 peaces?
Ive got vector of 5.1kb and 2 inserts of 1.2 eache
can any one tell me what ratio to use?
or if he ever secsead in any 3peace ligation?
Posted 24 July 2002 - 05:02 AM
Posted 24 July 2002 - 10:44 AM
thank alot for your advice its good to know that some done it before
i had traid 3 different ratio of insert and vector
1:3:3 1:6:6 1:10:10 and i did ligation for 2 houre in 24 celsius degree and overe night in 16 celsius degree
ill let you know what i've got
Posted 24 July 2002 - 10:51 AM
good luck, Dave.