I've tried to establish some cancer cell lines from whole tumor using different protocols as described elsewhere.
Now I've finally managed to isolate some cells growing in RPMI, but how do I actually know that it is cancer cells and not benign cells from normal healthy tissue? Can non-malignant cells grow at all in plain RPMI without growth factors etc?
Can I use some DNA-staining techniques for FACS?
Edited by Jojje, 20 December 2009 - 02:26 PM.