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Nonyl acridine orange (NAO) staining


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#1 cardosopedro

cardosopedro

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Posted 20 December 2009 - 04:49 AM

Hi.

I'm preparing a protocol to use this week. I will use nonyl acridine orange (NAO) to measure mitochondrial mass. I was reading some papers and a doubt came to my mind. In some papers the authors have done the NAO incubation at room temperature, while in other papers the incubation was done at 37 C... :rolleyes: :D Is there anyone with experience on this?

Thanks!

Edited by cardosopedro, 20 December 2009 - 04:50 AM.





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