how long is carb stable in liquid broth at 37C
Posted 18 December 2009 - 07:28 AM
I have transformed E.coli with a transformant which makes them grow slower compared to normal E.coli (I've seen that in agar plates). Because of this, the doubling times are increased. So when I want to make miniprep, I have to let them grow longer times (like 24-36 hrs at 37C). Here is my question: is carbenicillin stable in the broth at 37C for such a long time? I am concerned if the things which grow after 36 hrs might be contaminants.
Thanks in advance for your help!
Posted 18 December 2009 - 12:00 PM
Edited by sashay77, 18 December 2009 - 12:02 PM.