1 reply to this topic

[jangajarn]

Hi all,
   I have transformed E.coli with a transformant which makes them grow slower compared to normal E.coli (I've seen that in agar plates). Because of this, the doubling times are increased. So when I want to make miniprep, I have to let them grow longer times (like 24-36 hrs at 37C). Here is my question: is carbenicillin stable in the broth at 37C for such a long time? I am concerned if the things which grow after 36 hrs might be contaminants.
Thanks in advance for your help!

[sashay77]

Check the pH of your broth after 24 hrs, if it is not acidic, then carb should be stable... I am guessing you are using LB @ 7.4.

Edited by sashay77, 18 December 2009 - 12:02 PM.