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good housekeeping for ovary - PCR, RT-PCR and Real-Time PCR - BioForum

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good housekeeping for ovary


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2 replies to this topic

#1 majo

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Posted 18 December 2009 - 06:36 AM

Hi, I need some help
I have found some problems using 18s as a HKG in my primary cultures. Iím using some PKA activators and inhibitors and checking its effects we realized that ef-α was strongly affected by our treatments. We decided to perform qPCR using 18s, and we detected also strong differences between controls and treated groups. 18s is supposed to be a god HKG but in our model, we canít normalized with it.
Someone knows a better option for ovary?
Thanks!

#2 tea-test

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Posted 20 December 2009 - 09:31 AM

Hi, i found this article:

pubmed link

They analyzed normal and malignant ovary.
tea-test: The artist formerly known as Ned Land

#3 majo

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Posted 21 December 2009 - 01:38 AM

Thanks for helping
Majo




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