I try to make blots for MMP2 and MMP9 but I don't receive appropriate bands. In both cases I have bands on the same level: about 37 kDa ( like for GAPDH?) and no other bands. MMP2 should be at 72kDa and MMP9 92kDa.
I'm using Santa Cruz antibodies and for primary antibody dilution was 1:200, for secondary 1:500.
I'm using chemiluminescence for detection.
What went wrong?
Edited by Alsai, 17 December 2009 - 01:48 PM.