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CLED Agar Help Please

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#1 elaine



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Posted 17 December 2009 - 12:24 PM

I am a student at the University of Portsmouth studying part time for BSc in Applied Science. One of the units taken is clinical microbiology and I have a query relating to CLED agar.

I performed a disk diffusion test on a CLED agar plate inoculated with E.coli. A disk of Trimethoprim was added and incubated to observe growth. The antibiotic disk had some effect upton the growth but had not completely inhibited it - there was no clear zone of inhibition.

I was wondering if there was a certain substance in the CLED agar that contributed to this?


#2 pito



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Posted 18 December 2009 - 06:13 AM


what do you mean with there is no clear zone of inhibition?

I can see a zone of no growth, inhibition on the picture you included.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.

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