Site Directed methylation
Posted 17 December 2009 - 07:11 AM
I am new to the area of epigenetics and am planning some experiments related to the same. I am wondering if there are any methods similar to site-directed mutagenesis, so as to perform site directed methylation. Any information is appreciated.
Regards to all
Posted 17 December 2009 - 01:26 PM
we have been grappling with this problem also in the past,
have a look at NEB for Methyltransferases that block restriction sites ie: they recognise a particular restriction site and methylates it.
There are also non-specific methyltransferases including CpG methylase (SssI) and a GpC methylase too I recently found out.
Otherwise there are oligo synthesis companies that could generate an oligo for you with a methylated CpG site.
All comments and communication are my own personal ones, and are not tied to any of my affiliations.