Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Site Directed methylation

  • Please log in to reply
1 reply to this topic

#1 SDP



  • Members
  • Pip
  • 3 posts

Posted 17 December 2009 - 07:11 AM

Hello friends,
I am new to the area of epigenetics and am planning some experiments related to the same. I am wondering if there are any methods similar to site-directed mutagenesis, so as to perform site directed methylation. Any information is appreciated.

Regards to all


#2 methylnick



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 266 posts

Posted 17 December 2009 - 01:26 PM

Hi Sam,

we have been grappling with this problem also in the past,

have a look at NEB for Methyltransferases that block restriction sites ie: they recognise a particular restriction site and methylates it.

There are also non-specific methyltransferases including CpG methylase (SssI) and a GpC methylase too I recently found out.

Otherwise there are oligo synthesis companies that could generate an oligo for you with a methylated CpG site.

Good luck


All comments and communication are my own personal ones, and are not tied to any of my affiliations. 

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.