Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

primer design problem

  • Please log in to reply
1 reply to this topic

#1 skyenemy



  • Active Members
  • Pip
  • 6 posts

Posted 16 December 2009 - 08:49 AM

i am designing primer for SNP-RFLP and no restriction enzyme is available to cut the SNP site.
so, i make an artifical restriction site by inducing a primer mismatch in the primer, so that the pcr product can be cut by enzyme.

however, the mismatch i induced is near the SNP site, and after cutting by enzyme, the fragment is too short that cannot be visualized...
someone said i can add a poly(T) tail to the 5' end of the mismatch primer so that the fragment can be long enough.

anyone know whether it will work???

#2 phage434



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts

Posted 16 December 2009 - 09:15 AM

Yes, this will likely work. There is nothing magic about poly T -- an arbitrary sequence would also be fine (one that doesn't also get cut by your enzyme, of course).

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.