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Cell fixation with glutaraldehyde


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#1 Irene

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Posted 16 December 2009 - 06:19 AM

Hi, I am doing a staining of my cells with crystal violet that get into de cell and binds DNA. The fisrt step is to fixate my cells with glutaraldehyde 1% and then stain them. The thing is that I cannot do the staining till next day, so I wonder what is the best way to preserve my cells after fixation, with/without glut. in water...

Thanks¡¡

#2 bob1

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Posted 20 December 2009 - 03:59 PM

Crystal violet binds to protein as well as DNA so the staining may not be specific, unless you have a DNA specific protocol!

I stain and fix the cells in one step for viability/kill curve assays using crystal violet in 0.4% glutaradehyde. You should be able to keep the cells in the glut solution for about a week at 4 deg C.

#3 blotted

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Posted 21 December 2009 - 07:42 AM

Crystal violet binds to protein as well as DNA so the staining may not be specific, unless you have a DNA specific protocol!

I stain and fix the cells in one step for viability/kill curve assays using crystal violet in 0.4% glutaradehyde. You should be able to keep the cells in the glut solution for about a week at 4 deg C.



After the fixation you can keep your cells with PBS at 4ºC all the time you need. :D




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