1 reply to this topic

[anonymous]

I am stimulated cells with a recombinant protein. I would like to know if there is anyone who knows of a  way to detect this protein's subcellular localization with precision or if anyone has done similar type of expreriments? For example, is it possible to use GFP-fusion protein (my protein is about 21Kd) or is it better to use immunofluoresence?

Thanks

[anonymous]

Dmitris:

I suggest you label your protein with FS, a reagent available with Panvera Corporation, Madison, Wisconsin. It is a small fluorescent label that will not interfere with  your protein's solubility or function.

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