Posted 15 December 2009 - 02:13 AM
I see quite a lot of buffer questions, but this one has a bit different approach, I think. Ok, I'm supposed to make a buffer consisting of:
10 mM Na2HPO4, pH 7.2
20 mM NaCl
5 mM EDTA
25% (w/v) sucrose
I will be needing really small amounts of this, I'm thinking around 20 mL and will only use it once if everything goes as planned.
So I was thinking I'd do 100 mL in case I would need some more. This means I'd need:
0.117 g NaH2PO4*H2O
0.163 g Na2HPO4
0.0117 g NaCl
0.146 g EDTA
25 g sucrose
And to me, those amounts seem way to small for me to handle and to get close to anything accurate. But if I'd simply bring it up a factor 10 and dilute it later, I'd need alot of sucrose so I wouldn't want to do that either. If I'd save the sucrose, I wouldn't know how much water to use in the first place. Any tips?
Posted 15 December 2009 - 02:45 AM
Posted 15 December 2009 - 11:56 AM
I guess you could freeze whatever you're not using at -20 until needed.
Posted 17 December 2009 - 01:50 AM