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is it necessary to do DNases treatment?


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#1 wntiong

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Posted 14 December 2009 - 11:08 PM

i got low amount of rna for all my samples (less than 10ug), but got to save them for real time pcr and microarray. i heard it is important to do dnases treatment of sample before real time/conventional pcr to avoid genomic contamination, but after dnases treatment, the sample should be clean up which i think my rna will be lost some in this step. I design my primers span exon-exon junction, will that help to avoid genomic dna amplification if i don;t dnases my sample? thanks

#2 chrisbelle

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Posted 16 December 2009 - 12:47 AM

If you have exon-exon spanning primers then there's no need to do DNase treatment. 10ug? per uL or?

Chris
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#3 wntiong

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Posted 20 December 2009 - 08:36 PM

If you have exon-exon spanning primers then there's no need to do DNase treatment. 10ug? per uL or?

Chris


1ug/ul. need 10 ug in 10 ul for microarray. due to sample scarcity, hard to get this amount for my samples.




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