I want to use a in-vitro expression system for fast and high-thoughput protein expression.....
but these kit are quite expensive.....
do you know which one is the best ?
any one tried in-vitro expression system ?
1 reply to this topic
Posted 29 December 2009 - 04:49 AM
I'm using Ambion mMessage machine for RNA transcription and promega rabbit reticulocyte lysate system for in vitro translation. I've used it three times and got mixed results. I don't know if the problem lies with transcription or translation or my technique. Anyway my bit of opinion though.