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any one tried in-vitro expression system ?


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#1 lactamase

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Posted 14 December 2009 - 08:14 PM

I want to use a in-vitro expression system for fast and high-thoughput protein expression.....

but these kit are quite expensive.....

do you know which one is the best ?


Thx :lol:

#2 jangajarn

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Posted 29 December 2009 - 04:49 AM

I'm using Ambion mMessage machine for RNA transcription and promega rabbit reticulocyte lysate system for in vitro translation. I've used it three times and got mixed results. I don't know if the problem lies with transcription or translation or my technique. Anyway my bit of opinion though.




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