SDS-Page and DMSO
Posted 14 December 2009 - 01:09 PM
I would like to incubate a protein complexe in 70-90% DMSO and then separate it with SDS-PAGE. Somebody knows if DMSO can cause troubleshooting during electrophoresis?
Thank you for your answer.
Posted 14 December 2009 - 02:44 PM
I do not think you should have difficulty with electrophoresis. Pierce makes a kit (SDS-PAGE sample prep kit 89888) that uses DMSO in a protein binding buffer. The troubleshooting section says to increase [DMSO] to >60% to improve efficiency of binding. here is the link:
Posted 14 December 2009 - 03:09 PM