Hi,
I would like to incubate a protein complexe in 70-90% DMSO and then separate it with SDS-PAGE. Somebody knows if DMSO can cause troubleshooting during electrophoresis?
Thank you for your answer.
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#2
lab rat
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Posted 14 December 2009 - 02:44 PM
Hi Baeinoix,
I do not think you should have difficulty with electrophoresis. Pierce makes a kit (SDS-PAGE sample prep kit 89888) that uses DMSO in a protein binding buffer. The troubleshooting section says to increase [DMSO] to >60% to improve efficiency of binding. here is the link:
http://www.piercenet...les/1728dh5.pdf
regards,
lab rat
I do not think you should have difficulty with electrophoresis. Pierce makes a kit (SDS-PAGE sample prep kit 89888) that uses DMSO in a protein binding buffer. The troubleshooting section says to increase [DMSO] to >60% to improve efficiency of binding. here is the link:
http://www.piercenet...les/1728dh5.pdf
regards,
lab rat
42..."An immutable fixed-precision number of unlimited magnitude." <a href="http://en.wikipedia....amming_language)" target="_blank">http://en.wikipedia....amming_language)</a>, accessed 25June2009.
#3
bguyonnet
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bguyonnet
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Posted 14 December 2009 - 03:09 PM
Thank you for your answer but it seems that the DMSO is removed in the kit during wash step. I think I will remove DMSO by SpeedVac my samples...
