I am new in Y2H and doing the yeast transformation(Y2H gold strain from clontech) with my bait- pGBKT7.I am running the control all along.But the problem is my bait does not grow in SD/-trp DO plate.I am exactly following the yeastmaker yeast transformation system manual from clontech.Control is working well.So I am trying to resolve this issue.Please suggest me probable causes and basic tricks if any.Thank you.
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1 reply to this topic
Posted 27 December 2009 - 07:32 PM
I'm also using the clonetech system with pGBKT7 and pGAD vectors. For transformation, the GEITZ lab short protocol is working well with me, give it a try, its very quick but you gotta change it according to your own requirements.
I/we have to wait for 30 years to let the mirAcle haPPen....someone here told me!!!!