Taq at 2-8
Posted 14 December 2009 - 05:05 AM
I have a quick question about Taq DNA polymerase. I have Taq which has been accidently stored ( by my colleauge ) for about two months at 2-8 C instead of -20 C . Would you think it still can be used ? . Since Taq is a thermostable enzyme I assume it can withstand temperatures like 2-8 , at least for two months , though I am not sure . Anything of that sort of accident had happened to you someday ? Did the enzyme remain active with no problems or perhaps you had to use slightly greater amounts per reaction ?
Posted 14 December 2009 - 09:53 AM
Posted 14 December 2009 - 10:00 AM
Posted 14 December 2009 - 12:51 PM
Because currently we don't hae Taq stored in the refrigerator , It was the last tube of Taq we had . And We have just found this tube in the fridge . So I could get a false negative result because of the Taq having lost its efficiency , or I could get a true negative result and there's no way to tell which one is the case . Besides, I need to know quickly.
Why don't you just do a reaction with the Taq stored in the refrigerator and another with an aliquot from the freezer and get the answer ??
Posted 14 December 2009 - 12:52 PM
Thank you. It does make sense . I hope It'll be ok.
An unopened tube of Taq DNAP is stable on the benchtop (room temperature) for 6 months. The reason we keep it at -20C is to prevent the growth of bacteria and mold once the tube has been opened.