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keratinocyte culture


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#1 grace.tan

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Posted 13 December 2009 - 07:20 PM

Hi,

I've been culturing keratinocytes using ATCC media, but I have a problem of contamination after about one week of culture. The contamination does not turn the media cloudy or give a smell, but looks like cell debris.



Has anyone encountered this before?

#2 aimikins

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Posted 14 December 2009 - 12:54 PM

I'd test for mycoplasma.

also, do everything you can to rule out other contaminants, i.e. go over your technique and materials. do you change the filter in your auto-pipettor? etc.

is it possible for you to post a high-res pic of your plate with and without the debris?
"it is a miracle that curiosity survives formal education" -A.E.




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