bicarbonate in cell culture: powder v. liquid?
Posted 13 December 2009 - 12:37 PM
The basic question is: can I add the bicarbonate just as a powder to the liquid medium? Or do I need to dissolve it in something first and then add?
Sorry for the silly question, I'm new to cell culture.
Posted 13 December 2009 - 12:42 PM
Posted 14 December 2009 - 07:43 AM
but, addition of powder may affect the pH of your medium.
you can prepare and adjust a solution of bicarbonate prior to addition to your medium or you can add powder and check and adjust the pH, as necessary (you may not be adding enough to have a significant effect).
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 20 December 2009 - 01:38 AM
Adding it can contaminate your medium.
I'd dissolve it first, sterilize it and then add it to the medium.