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Probe preparation for gel shift


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#1 anonymous

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Posted 03 December 2001 - 10:00 PM

Hi,

I am interested in finding out if anyone has had success using a PCR product as a probe for gel shift?Also, what are some of the other types of probes people have had success with?Thanks in advance



#2 J

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Posted 15 January 2002 - 09:23 AM

PCR products have worked for me, just don't forget to consider how youy are going to label the product ie include a restriction site in one primer. I've also used synthetic oligos that I annealed together and then labelled.




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