Posted 13 December 2009 - 09:19 AM
I cloned my genes into pGEX-2T expression vector. I checked my inserts via colony PCR and the results are okey. But when I digest the plasmid to see my insert, it becomes a problem. There is something at expected level, but it is not an exact band, something imaginary. I checked all my stocks, primers, transformed it (I used BL21 compotent cell, because I will use the vectors in protein purification. Does it affect restriction enzyme efficiency?) again, but all same. I cannot solve the problem. Any help would be appreciated.
Posted 13 December 2009 - 10:04 AM
Posted 13 December 2009 - 10:59 AM
Posted 13 December 2009 - 01:56 PM