1 reply to this topic

[qtpy]

Hello,

I'm trying to learn how the acid dissociation procedure works in developing the immunogenicity assay (ELISA)? What does it mean by dissociation and neutralization?

Thank you for your help in advance

[CellSpecific.com]

Here are some links to help you:

Ref: "An acid dissociation bridging ELISA for detection of antibodies directed against therapeutic proteins in the presence of antigen."
http://www.ncbi.nlm....pubmed/16107258

Nice schematic on use of acid dissociation of complexes:
http://www.emea.europa.eu/pdfs/conferencef...mwp/swanson.pdf

Article describing use of method in evaluating Clinical Immunogenicity of Panitumumab
http://www.jimmunol....ull/178/11/7467

Definition:
Dissociation: Molecular entities move from being physically associated to being separated. Separation can be a normal event or induced by disrupting electrostatic/h-bonding interactions.
Neutralization: Many meanings for this; from antibody perspective, antibody can neutralize simply by blocking its target antigen from binding the antigen's partner molecule (or receptor). In the same way, a soluble antigen could neutralize antibody binding to the preferred immobilized (cell surface or plate coated antigen) target antigen.

Hope this helps.

Edited by CellSpecific.com, 13 December 2009 - 12:18 PM.