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cell adhesion assay


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#1 SF_HK

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Posted 11 December 2009 - 08:26 PM

Hi,

I plan to do a cell adhesion assay (non flurescent) soon for my parental cell line and cell line transfected with gene of interest. I have no experience doing this and thus all suggestions would be welcomed.

I have recently ordered collagen but can someone share with me on how to pre treat the plates with collagen and ths standard procedure to carry out the assay.

#2 jangajarn

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Posted 29 December 2009 - 04:54 AM

check out Cell-Cell Interactions Methods and Protocols and Adhesion_Protein_Protocols from Methods in Molecular Biology.. you'll get all varieties of cell adhesion assays




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