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freezing during RNA isolation step


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#1 wjchxl

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Posted 11 December 2009 - 01:08 PM

Hi,

I am using RNeasy kits from Qiagen for RNA isolation. I am wondering if the samples could be frozen down after lysis step. If yes, stored at what temperature? What risks are associated for long-term storage?

Thanks!

#2 gogreen

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Posted 13 December 2009 - 09:40 AM

yes..The cell/tissue lysates in RLT buffer (with B-ME added) can be stored at -70 for quite long times (I've tried upto 3 months without any significant degradation)...or at 4 degrees for a week to month and above or at RT for 3-4 days (the RT option is not a good choice unless u want to ship it somewhere...for storage, the -70 is the best!!)

Edited by gogreen, 13 December 2009 - 09:41 AM.





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